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Expression in Pseudomonas putida of chiA from Serratia marcescens gave improved protection of beans against Sclerotium rolfsii (Chet et al., 1993). Dunne et al. (2000) showed that overproduction of an extracellular serine protease by Stenotrophomonas maltophilia W81M3 or W81M4 resulted in improved control of Pythium damping-off of sugar beet by the recombinant strains as compared to the wild-type strain W81.

Induced resistance

Introduction of pchCBA from Pseudomonas protegens CHA0 (formerly Pseudomonas fluorescens) into strain P3 enabled salicylic acid production and improved the ability of P3 to induce systemic resistance in tobacco against tobacco necrosis virus (Maurhofer et al., 1998).

Antibiosis/toxin production

Transfer and expression of the HCN biosynthesis operon hcnABC from Pseudomonas protegens CHA0 into Pseudomonas fluorescens P3 resulted in improved control of black root rot of tobacco by the transgenic strain (Voisard et al., 1989).

Transfer of a recombinant plasmid pCU203, containing genes for the biosynthesis of 2,4-diacetylphloroglucinol (DAPG) cloned from Pseudomonas sp. F113, into Pseudomonas sp. strain M114 yielded M114(pCU203), which gained the ability to synthesise DAPG and control Pythium ultimum damping-off of sugar beet better than did M114 (Fenton et al., 1992).

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