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A short course of lectures
«The Impact of Food Bioactives on Health»

Read-Out of the System and How This Information Can Be UsedGastric MotilityAdvantages, Disadvantages and Limitations of the SystemRelevance to Human In Vivo SituationThe Simulator of the Human Intestinal Microbial Ecosystem (SHIME®)Description of the VTT One Compartment Fermentation ModelStatic MethodsThe Cell Culture HoodApproaches to Static Digestion ModelsHistory and Special Features of the ModelStability and Reproducibility of the SystemPharmaceutical-Based ResearchFeatures and MechanismsUssing ChamberApplications: Advantages and DisadvantagesRole of Food Material PropertiesExperimental ReadoutThe DIDGI® SystemThe IPEC-J2 Cell LineDigestion ConditionsOrigin and Some Features of THP-1 and U937 CellsQuality in Relation to Other Models with the Same ApplicabilityT Cell Isolation ProtocolsOrigins and Design of the DGMComparison In Vitro/In Vivo DataModel DescriptionUses of the DGMOriginOrigin, Features and MechanismsMonitoring ViabilityValidation of the SystemOriginCytokine ProductionDendritic CellsHistory of the ModelRelevance to Human In Vivo SituationFeatures and MechanismsStability and Reproducibility of the System-D Culture SystemsTIM-1Inoculation of Gut Fermentation ModelsAdvantages, Disadvantages and LimitationsRelevance to Human In Vivo SituationTrypsinisation and Subculturing of CellsPreparation of a Food BolusGeneral ProtocolReadout of the Porcine Ex Vivo Intestinal Segment ModelCritical NotesMedium QualityConcept of TIMT Lymphocytes or T-CellsRelevance to Human In Vivo SituationThe Computer-Controlled Multicompartmental Dynamic Model of the Gastrointestinal System SIMGISpecial Features/Morphology/ReceptorsRelevance to the Human Situation (L-Cell In Vivo)Basic ProtocolUses of the HGSGeneral ProtocolOther Models with the Same ApplicabilityStability/Consistency/ReproducibilityCell Viability TestingDifferentiation of THP-1 and U937 Monocytes into MacrophagesDC SubsetsInfoGest Consensus MethodMonocyte-Derived DCsReadout of the Ussing Chamber SystemGeneral Protocol of Culturing THP-1 CellsThe Small Intestinal PhaseContamination of Cell CulturesFeatures and MechanismsStability, Consistency and ReproducibilityExperimental ReadoutRelevance to Human In Vivo SituationValidation of DIDGI® for the Digestion of Infant FormulaUssing ChamberAdvantages, Disadvantages and Limitations of the SystemCaco-2/Human Monocyte Derived DCs (Soluble Factors)Advantages and DisadvantagesFeatures and MechanismsGeneral Advantages and Disadvantages of the ModelExperimental Read OutVerification of Monolayer Integrity by Lucifer Yellow FluxCritical NotesEverted SacCo-stimulationAdvantages, Disadvantages and Limitations of the SystemIntestinal Crypt Organoids as Experimental ModelsCell Maintenance ProtocolCD34+-Derived DCsStability, Consistency and ReproducibilityGeneral ProtocolExperimental Protocol for Test CompoundsStability/Consistency and ReproducibilityMurine GLUTag CellsIn Vitro Studies on Food Bioactives Using DCs (Table 17.3)Advantages and LimitationsGastrointestinal OrganoidsAdvanced Gastric Compartment (TIM-agc)Static Models for Lipid HydrolysisStudy of Inflammatory ResponsesRelevance to Human In Vivo SituationFeatures and MechanismsControls to Test Stability and Performance of the ModelStability, Consistency and Reproducibility of the SystemAvailability of the SystemPorcine Ex Vivo Intestinal Segment ModelIncubation with Food Components and Digested Food SamplesProtein QualitySerum AdditionPrediction of Glycemic ResponsePeripheral Blood Mononuclear CellsRelevance to the Human L-Cell In VivoDynamic Gastric Model (DGM)Read-Out of the SystemGeneral ProtocolMonitoring ViabilityDendritic CellsAssess ViabilityQuality in Relation to Other Models with the Same ApplicabilityGeneral ProtocolThe TNO In Vitro Model of the Colon (TIM-2)Assess ViabilityCalculating Transepithelial ResistanceBasic ProtocolGeneral ProtocolFeatures and MechanismsRole of ACW Activity on Food DigestionThe Oral PhaseRead Out of the SystemGeneral ProtocolExperimental ReadoutAssess ViabilityGeneral ProtocolGeneral Advantages and Limitations of Intestinal Organoid SystemsGeneral MaintenanceHuman Gastric Simulator (Riddet Model)Description of TIM-2Monocytes and MacrophagesSmall Intestinal Crypt IsolationIn Vivo Digestion of Infant Formula on PigletsExperimental Read OutDC Cell LinesRelevance to Human In Vivo SituationGeneral ProtocolMicroorganisms Survival, Adhesion or InvasionIsolating Primary DCs from BloodExperimental ReadoutTroubleshooting Guide for Transport Experiments Across Caco-2 MonolayersOriginHuman Peripheral Blood Mononuclear CellsOriginStability/Consistency/Reproducibility of the SystemSamplingOriginCell Maintenance ProtocolControls to Test Stability and Performance of the ModelDescription of the ModelAdvantages, Disadvantages and Limitations of the SystemAnalysis of HGS Biomechanical RelevanceGastric ProcessingGeneral ProtocolOther DC ReadoutsProtocol for Polarizing Caco-2 Cells in Tissue Culture InsertsApplicationsQuality in Relation to Other Models with the Same ApplicabilityRelevance to Human In Vivo SituationSTC-1 CellsSpecial FeaturesGeneral ProtocolStudy of Proliferative/Cytotoxic ActivityGeneral Protocol for HT29-MTX CellsCaco-2/PBMCs (Soluble Factors)Features and MechanismsAdvantages, Disadvantages and LimitationsCaco-2/THP-1 (Soluble Factors)The TNO Gastro-Intestinal Model (TIM)Use of Digested Food SamplesPhysical ForcesCaco-2/B CellsStability and Reproducibility of the SystemRead-Out of the SystemHost-Gut Microbiota InteractionHT29 Cell LineAssess ViabilityGeneral ProtocolControls to Test Viability and Performance of the ModelAdvantages, Disadvantages and LimitationsOrigins of the HGSGeneral Protocols for Caco-2 CellsThe Ussing ChamberMonitoring ViabilityCo-cultivation of Caco-2 and HT-29MTXSample PreparationIntestinal Rings and Intestinal SegmentsOrigin, Features and MechanismsControls to Test Stability and Performance of the ModelControls: Positive and NegativeFood-Based ResearchRegulation of GLUTag Cell Secretory Activity by NutrientsCo-culture Caco-2 and B-cells (Raji)History of the ModelSample AnalysisOriginIndirect Positive Isolation of Human CD4+ TFreezing CellsSmall Intestinal Organoid PassagePerformance of Secretion and Expression AssaysAvailability of the SystemStability, Consistency and ReproducibilityThe Use of TIM to Study the Bio-accessibility of NutrientsCaco-2 Cell LineTiny TIMApplications of T Cell CulturesThe Porcine Ex Vivo Intestinal Segment ModelQuality in Relation to Other Models with the Same ApplicabilitySolubility/DialyzabilitySpecial Features of the ModelSpecial Features of the ModelStability, Consistency and ReproducibilityExperimental Read outGastric SecretionsThe Gastric PhaseHistory of the ModelDynamic Fermentation ModelsGeneral ProtocolFeatures and MechanismsCo-culture Caco-2 and Dendritic CellsStatic Models for Protein HydrolysisTemperature ControlGeneral Protocol for DGM ExperimentsSmall Intestinal Organoid CultureRelevance to Human In Vivo SituationRegulation of GLUTag Cell Secretory Activity by Other SignalsMaintenance of the GLUTag Cell LinePBMC-Derived T CellsGastric CompartmentExperimental ReadoutsCells Present in the IntestineNCI-H716 CellsPassaging of Cells in Suspension CultureCulture ConditionsAssess ViabilityOne Compartment Fermentation ModelDescription of SHIME®OriginRole of MicrobiotaSamplesFunctionality StudiesRelevance to Human In Vivo SituationCell Maintenance ProtocolIsolated and Perfused Intestinal SegmentsRead-Out of the System and How This Information Can Be UsedOrigins and Design of the DIDGI® SystemGeneral ProtocolsStability, Consistency and ReproducibilityGeneral ProtocolProtocol for the In Vitro Dynamic Digestion of Infant Formula Using the DIDGI® SystemAsses ViabilityLipidsGeneral Advantages, Disadvantages and Limitations of the Ussing Chamber SystemGeneral Cell Culture ProtocolsBiochemistryCo-culture of Caco-2/Human Monocyte Derived DCs (Include Contact Dependent Events)Experimental Protocol for Test CompoundsThe Thawing and Recovery of CellsExperimental ReadoutTransport StudiesExperimental Protocol for Test CompoundsOriginpH and CO2Co-culture Caco-2/Immune CellsExperimental Readout of the SystemPracticalitiesAdvantages, Disadvantages and LimitationsOther Static ModelsGastric EmptyingAssess ViabilityCell Culture Terminology (Fig. 1)Relevance to Human In Vivo SituationAssess ViabilityOperating ProtocolMeasurement of Transepithelial Electrical Resistance (TEER)General ProtocolDifferentiation of THP-1 and U937 Monocytes into Dendritic CellsRelevance to the Human In Vivo Situation
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