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A short course of lectures
«The Impact of Food Bioactives on Health»

OriginExperimental ReadoutStability, Consistency and Reproducibility of the SystemStability, Consistency and ReproducibilitySamplingSTC-1 CellsLipidsReadout of the Ussing Chamber SystemIsolated and Perfused Intestinal SegmentsMurine GLUTag CellsRelevance to Human In Vivo SituationGeneral Advantages and Disadvantages of the ModelStudy of Inflammatory ResponsesRelevance to the Human L-Cell In VivoStability, Consistency and ReproducibilityBasic ProtocolOriginCell Maintenance ProtocolStability, Consistency and ReproducibilityHuman Peripheral Blood Mononuclear CellsStatic MethodsGeneral Protocol for DGM ExperimentsGeneral Protocols for Caco-2 CellsSerum AdditionPerformance of Secretion and Expression AssaysDescription of the VTT One Compartment Fermentation ModelSmall Intestinal Crypt IsolationOrigins and Design of the DGMDifferentiation of THP-1 and U937 Monocytes into Dendritic CellsHuman Gastric Simulator (Riddet Model)Small Intestinal Organoid CulturePBMC-Derived T CellsRead-Out of the System and How This Information Can Be UsedIncubation with Food Components and Digested Food SamplesMonitoring ViabilityCaco-2/B CellsGeneral ProtocolOriginCaco-2/Human Monocyte Derived DCs (Soluble Factors)Controls: Positive and NegativeHT29 Cell LineCaco-2 Cell LineOriginTemperature ControlGeneral ProtocolT Lymphocytes or T-CellsOrigins and Design of the DIDGI® SystemExperimental Readout of the SystemCell Culture Terminology (Fig. 1)General Protocol-D Culture SystemsThe Oral PhaseOrigin, Features and MechanismsExperimental ReadoutAdvanced Gastric Compartment (TIM-agc)Special Features of the ModelControls to Test Stability and Performance of the ModelRelevance to Human In Vivo SituationUssing ChamberApplicationsDC Cell LinesRelevance to the Human In Vivo SituationExperimental Protocol for Test CompoundsRole of Food Material PropertiesStatic Models for Lipid HydrolysisIn Vitro Studies on Food Bioactives Using DCs (Table 17.3)Features and MechanismsGastric ProcessingSample AnalysisThe Use of TIM to Study the Bio-accessibility of NutrientsRelevance to Human In Vivo SituationAdvantages, Disadvantages and LimitationsOperating ProtocolUssing ChamberDC SubsetsContamination of Cell CulturesStability/Consistency and ReproducibilityGeneral ProtocolHost-Gut Microbiota InteractionCells Present in the IntestineMonitoring ViabilityMedium QualityGeneral Protocol for HT29-MTX CellsRelevance to Human In Vivo SituationGeneral ProtocolStatic Models for Protein HydrolysisFeatures and MechanismsFreezing CellsCaco-2/THP-1 (Soluble Factors)Description of SHIME®T Cell Isolation ProtocolsCo-culture of Caco-2/Human Monocyte Derived DCs (Include Contact Dependent Events)Experimental Protocol for Test CompoundsStability and Reproducibility of the SystemDendritic CellsHistory of the ModelModel DescriptionOriginRelevance to Human In Vivo SituationTroubleshooting Guide for Transport Experiments Across Caco-2 MonolayersGeneral ProtocolDendritic CellsPorcine Ex Vivo Intestinal Segment ModelNCI-H716 CellsAdvantages and DisadvantagesDescription of TIM-2Tiny TIMCritical NotesDifferentiation of THP-1 and U937 Monocytes into MacrophagesOriginFood-Based ResearchValidation of DIDGI® for the Digestion of Infant FormulaSample PreparationThe TNO In Vitro Model of the Colon (TIM-2)Gastric SecretionsCD34+-Derived DCsAdvantages, Disadvantages and LimitationspH and CO2One Compartment Fermentation ModelCo-culture Caco-2/Immune CellsOther DC ReadoutsThe Small Intestinal PhaseOrigin, Features and MechanismsMonitoring ViabilityGeneral MaintenanceExperimental ReadoutGeneral ProtocolExperimental Read OutRead-Out of the System and How This Information Can Be UsedThe Ussing ChamberAssess ViabilityAdvantages, Disadvantages and Limitations of the SystemCritical NotesStability, Consistency and ReproducibilityTrypsinisation and Subculturing of CellsFeatures and MechanismsSpecial Features of the ModelQuality in Relation to Other Models with the Same ApplicabilityStability, Consistency and ReproducibilityCell Viability TestingApplications: Advantages and DisadvantagesSpecial Features/Morphology/ReceptorsGeneral ProtocolGeneral ProtocolAvailability of the SystemAssess ViabilityFeatures and MechanismsAdvantages, Disadvantages and Limitations of the SystemMonocytes and MacrophagesProtocol for Polarizing Caco-2 Cells in Tissue Culture InsertsRelevance to Human In Vivo SituationExperimental Read OutCo-culture Caco-2 and B-cells (Raji)InfoGest Consensus MethodAdvantages, Disadvantages and LimitationsThe TNO Gastro-Intestinal Model (TIM)Assess ViabilityRole of MicrobiotaGeneral ProtocolSolubility/DialyzabilityAssess ViabilityControls to Test Stability and Performance of the ModelRelevance to Human In Vivo SituationThe Computer-Controlled Multicompartmental Dynamic Model of the Gastrointestinal System SIMGIAsses ViabilityGeneral ProtocolIntestinal Rings and Intestinal SegmentsExperimental ReadoutsSmall Intestinal Organoid PassageThe Thawing and Recovery of CellsRelevance to the Human Situation (L-Cell In Vivo)General ProtocolThe Cell Culture HoodGeneral Advantages and Limitations of Intestinal Organoid SystemsValidation of the SystemThe IPEC-J2 Cell LineIntestinal Crypt Organoids as Experimental ModelsGeneral ProtocolStability/Consistency/ReproducibilityDynamic Gastric Model (DGM)Protein QualityFeatures and MechanismsBasic ProtocolHistory of the ModelUses of the HGSGastric MotilityRead Out of the SystemRead-Out of the SystemAssess ViabilityOther Models with the Same ApplicabilityOriginMeasurement of Transepithelial Electrical Resistance (TEER)Advantages, Disadvantages and Limitations of the SystemReadout of the Porcine Ex Vivo Intestinal Segment ModelGeneral Advantages, Disadvantages and Limitations of the Ussing Chamber SystemOther Static ModelsPhysical ForcesAssess ViabilityStability/Consistency/Reproducibility of the SystemBiochemistryThe Gastric PhaseConcept of TIMCo-cultivation of Caco-2 and HT-29MTXCytokine ProductionSpecial FeaturesFeatures and MechanismsGeneral Cell Culture ProtocolsExperimental Protocol for Test CompoundsApplications of T Cell CulturesQuality in Relation to Other Models with the Same ApplicabilityIsolating Primary DCs from BloodCaco-2/PBMCs (Soluble Factors)Relevance to Human In Vivo SituationAnalysis of HGS Biomechanical RelevanceStability and Reproducibility of the SystemCulture ConditionsPrediction of Glycemic ResponsePracticalitiesPassaging of Cells in Suspension CultureMonocyte-Derived DCsCell Maintenance ProtocolOrigins of the HGSUses of the DGMCo-stimulationRole of ACW Activity on Food DigestionVerification of Monolayer Integrity by Lucifer Yellow FluxUse of Digested Food SamplesGeneral ProtocolControls to Test Stability and Performance of the ModelAdvantages, Disadvantages and Limitations of the SystemDynamic Fermentation ModelsComparison In Vitro/In Vivo DataFeatures and MechanismsGeneral ProtocolAssess ViabilityCalculating Transepithelial ResistanceHistory and Special Features of the ModelRead-Out of the SystemGastric EmptyingRegulation of GLUTag Cell Secretory Activity by NutrientsControls to Test Viability and Performance of the ModelPeripheral Blood Mononuclear CellsIn Vivo Digestion of Infant Formula on PigletsOriginRelevance to Human In Vivo SituationStudy of Proliferative/Cytotoxic ActivityPreparation of a Food BolusMicroorganisms Survival, Adhesion or InvasionAdvantages and LimitationsAvailability of the SystemIndirect Positive Isolation of Human CD4+ TPharmaceutical-Based ResearchInoculation of Gut Fermentation ModelsRegulation of GLUTag Cell Secretory Activity by Other SignalsStability and Reproducibility of the SystemProtocol for the In Vitro Dynamic Digestion of Infant Formula Using the DIDGI® SystemOrigin and Some Features of THP-1 and U937 CellsHistory of the ModelAdvantages, Disadvantages and LimitationsExperimental ReadoutFeatures and MechanismsThe Simulator of the Human Intestinal Microbial Ecosystem (SHIME®)Experimental ReadoutSamplesCell Maintenance ProtocolQuality in Relation to Other Models with the Same ApplicabilityGastric CompartmentQuality in Relation to Other Models with the Same ApplicabilityDigestion ConditionsCo-culture Caco-2 and Dendritic CellsRelevance to Human In Vivo SituationEverted SacGeneral Protocol of Culturing THP-1 CellsRelevance to Human In Vivo SituationGeneral ProtocolsExperimental Read outTransport StudiesApproaches to Static Digestion ModelsThe Porcine Ex Vivo Intestinal Segment ModelTIM-1The DIDGI® SystemGastrointestinal OrganoidsDescription of the ModelMaintenance of the GLUTag Cell LineFunctionality Studies
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