An optical tweezer system formed by a focused beam of LP2i mode in fiber was demonstrated to be a simple and efficient method in the manipulation of bioparticles, including cell pairing, separation or re-grouping by selective translation of captured cells, and the rotation of cell clusters. Translational was estimated to be 0.84 pN experimentally, in comparison with 1.2 pN modeled by using geometric optics (RO model) and a Gaussian approximation of beam lobes. This all-fiber single-probe optical chuck has the advantages of (1) being a robust, easy-to-implement single fiber
probe; (2) peak irradiance on target particles are significantly lower than multi-beam tweezers using focused Gaussian beams, thus reducing the risk of target damage from high intensity light; (3) LP21 mode can be generated across a wide range of wavelength; (4) LP21 mode fiber optical chuck are capable of performing manipulation with translation, curved motion, and rotation. Such a system has many applications in individual cell sorting/filtering, and orientation control in various biomedical testing and diagnostic systems.