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Fundamental Theory

Extracting Sugar from Agricultural Wastes

The goal of this chapter was to effectively decompose waste bagasse cellulose with a biological method for reducing the amount of sugar used as a nutrient in hydrogen-producing bacteria. Figure 1 is the experiment flow chart for this chapter. This research tried to develop a method to decompose cellulose in agricultural waste (Figure 2) to extract sugar as the substrate for the bio-hydrogen production system.

Experiment flow chart of bio-hydrogen production experiment

Figure 1. Experiment flow chart of bio-hydrogen production experiment.

Cane fiber without treatment and post-treatment

Figure 2. Cane fiber without treatment and post-treatment.

The composition and structure of lignocelluloses is discussed in the first part. The resources and characteristics of bacteria and required growth elements for cellulose bacteria cultivation and generation are explained in the second and third parts.


Lignocelluloses is regarded as the most abundant biomaterial, as it comes from many sources including agricultural residuals and wastes (bagasse, hull, stalk, and vegetable residuals), forest wastes (branches and wooden meal), and solid wastes from cities and towns. Cheap and sufficient materials are therefore available [13]. Lignocelluloses are composed of cellulose, hemicellulose, and lignin, which are also the major components of recycling resources [14]. They can be combined with other polysaccharides, such as xylan, mannan, polyuronides, and some galactans [15]. We acquired bagasse from the Lioho Tourist Night Market, the skin was removed to obtain soft sugarcane fiber, cut it into 5 cm sections, and reduced to powder with a homogenizer. After passing it through a No. 10 sieve, powder was baked at 80 °C for 10 hr.

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