Thermostable Cellulolytic Bacteria
Tai (2004)  separated thermostable cellulose bacterium T4 from compost from Taiwan Sugar Corporation and compared it with other cellulose bacteria. He found that domestic cellulose bacteria T4 effectively decomposed cellulose at 60 °C and transformed it into sugar (Figure 3). The high temperature could be utilized by industries to enhance the mass production of sugar with cellulose hydrolysis. This experiment therefore utilized bacteria strain T4 to transform cellulose into sugar for further hydrogen production. The 60 °C growth temperature is suitable for the high-temperature environments of industry. Bacteria strains for the present study were purchased from Bioresource Collection and Research Center (BCRC), numbered BCRC 17200.
Nutrient agar broth and bagasse broth were also introduced into the growth medium. The major difference is that nutrient agar broth was used for initial T4 activation and growth. The growth medium contained rich nutrition and was suitable for the growth and activation of bacteria strains. On the other hand, bagasse broth was used for effectively transforming bagasse cellulose, the major nutrition source of T4, into sugar.
Figure 3. G. Thermostable Cellulolytic Bacteria T4.
Reduced Sugar in the Sample
To appropriately dilute the sample solution, 0.5 ml of the solution and 0.5 ml of DNS indicator were heated in a water tank at 100 °C for 5 min until the color of the indicator changed. The solution was then cooled in a cold-water bath, 3 ml of distilled water added, and a spectrophotometer measuring a wavelength of 540 nm utilized to measure absorbency. The amount of reduced sugar in the sample was calculated by comparison to the absorbency-to- glucose curve.