Home Economics American Trypanosomiasis Chagas Disease, Second Edition: One Hundred Years of Research
Although a great variety of animal models could be used as experimental models for the study of Chagas disease, the reproducibility in experimental conditions of all histopathological and clinical manifestations of Chagas disease observed in humans is not possible in all species. Unfortunately, the studies are not performed in standardized conditions even when the same animal species is considered, with regards to race; genetic background; T. cruzi strain; source of the inoculum; inoculum; number of inoculations; route of inoculation; age, sex, and weight of the animal; parameters of evaluation; and length of infection. Moreover, when studies using the same T. cruzi strains in similar conditions in different animal species show that the results observed are not similar, it suggests that it is not possible to extrapolate experimental results from one animal model to another.
Thus, the choice of an experimental model is dependent on the subject to be investigated and the previous knowledge acquired throughout time after one century of research related with this theme. A new system based on a highly sensitive in vivo imaging system of genetically modified T. cruzi bioluminescent may be capable of expressing luciferase open perspectives for both better comprehension of the pathogenesis of Chagas disease in distinct animal models as well as evaluation of therapeutic efficacy of current and new drugs.
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