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Multilocus sequence typing (MLST)

The use of multilocus sequence typing (MLST) has increased in tandem with whole genome sequencing in order to answer many questions of LAB relatedness and evolution (Enright and Spratt, 1999; Maiden et al., 1998; Sun et al., 2014). MLST relies on DNA sequence analysis of conserved housekeeping genes (or other protein-coding sequences) to type bacteria (Enright and Spratt, 1999; Maiden, 2008) and reveal insight into the overall diversity of a species. MLST has direct appeal to the brewing industry not only because of lower cost and required time compared to whole genome sequencing, but also due to the potential of distinguishing same-species isolates recovered from different sources and thereby the potential influence of the beer niche on genetic adaptations. However, in order to effectively develop MLST into a rapid means of screening for BSR versus non-BSR LAB, whole genome data provided by deep sequencing needs to be available to inform on specific assay targets.

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